Genetic Engineering

Course Outline: (34 hours)

Contents:

1. Background, importance, position and applications of genetic engineering

2. Carriers: plasmids and bacteriophages

3. Extraction and purification of DNA from living cells

4. Manipulation of purified DNA and use of important enzymes

5. Introduction and enter of DNA molecule into living cells

6. Colonization carriers for colibacillosis and some other creatures

7. Methods to obtain clone from pure gene

8. Evaluation of gene location and structure

9. Evaluation of gene expression

10. Monoclonal antibodies

11. Production of protein from colonized genes

12. Genetic engineering in medicine, as well as food and medication industries

13. Transgenic animals and plants and their medical applications